NMRlab
  • Login & order NMR service now
  • NMR service
    Login & order NMR service now NMR service NMR chromatography service Why use our superior service Contact us The NMR team How to submit samples Use the instruments yourself Terms & conditions
  • Nuclear Overhauser …
  • NMR
    Go to home page What is NMR Techniques Apps Guides Contact us
  • עברית
  • HU NMR lab
  • Login & order NMR service now
  • NMR service
    Login & order NMR service now NMR service NMR chromatography service Why use our superior service Contact us The NMR team How to submit samples Use the instruments yourself Terms & conditions
  • Nuclear Overhauser …
  • What is NMR
    What is NMR Uses of NMR Basis of NMR Chemical shift Spin-spin coupling
  • Techniques
    Techniques 1H NMR 2D NMR Relaxation Multinuclear Semi-solids Solid state
  • Apps
    Apps Solvent shifts NMR thermometer Reference frequency
  • Guides
    Guides Measuring a 1H spectrum on the old 500 Measuring a 1H spectrum Measuring other nuclei Measuring 2D NMR Measuring diffusion Measuring relaxation Measuring solid & semi-solid
  • Contact us
  • Terms & conditions
  • עברית

Nuclear Overhauser effect (NOE)

When a specific nucleus is magnetically excited and its neighbor is at equilibrium, relaxation occurs between the two nuclei. Because of this relaxations, the intensity of the nucleaus that was at equilibrium changes. For a carbon and its neighboring proton it is possible to observe an almost four-fold increase in signal stranght resulting from decoupling. On the other hand in the 15N spectrum, the effect is negative and attenuates the 15N signal by 89%.

The smaller the physical distance between the equilibrium nucleus and the excited nucleaus, the bigger the expected change. The change for a carbon, two bonds away from the proton is about 3% by comparison to 297% for a single bond. This dependence can be used to estimate the distance between nuclei in a 2D experiment such as NOESY or ROESY.