The analytical Chemistry Lab
Unit
Description
Our
laboratory is an analytical unit, which provides Elemental Analysis and LCMS Analysis
service for universities, chemical and pharmaceutical companies, research and
industrial foundations. We perform the following evaluations: analysis of
C, H, N and O, the halogens: Cl,Br,I,F
and S,P, all in a broad spectrum of samples, (for example, synthesized organic
compounds, natural products, coal and coal derived materials, pharmaceutical,
industrial samples and others), High resolution mass determination, detection
and separation in a wide range of polar masses such as: small molecules, proteins,
peptides and oligonucleotides
Methods and Equipment
Elemental analysis
1. Determination of C,
H, N and O is performed using the Thermo Flash
2000 CHN-O Elemental Analyzer. It uses a simultaneous
flash combustion method (950-10600C)
for CHN and pyrolysis of oxygen to
convert the sample elements to simple gases. The system uses a steady-state,
wave front chromatographic approach to separate the controlled gases. They are
detected as a function of their thermal conductivity. The analyzer uses a globally-recognized technique
that is endorsed by official organizations worldwide. High speed
microprocessor control, solid-state components and built-in diagnostics, provide
assurance of performance fast,
reliable, and accurate results for carbon, hydrogen, nitrogen, and oxygen detection.
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CHN-O Elemental Analyzer 2.
Determination of halogens is
done using the oxygen-flask combustion
method (Schoniger application) or using the Anton Paar Microwave Induced Oxigen Combustion
(MIC) for the decomposition of organic samples and subsequent potentiometric titration by the 835 Titrando
Metrohm Titroprocessor and by Ion chromatography analysis using a Dionex
IC system. 3.
Determination of sulfur is done via oxygen-flask combustion method or using the Anton Paar Microwave Induced Oxigen Combustion
(MIC) for the decomposition of organic samples and subsequent gravimetric titration and by Ion chromatography
analysis using a Dionex IC system. 4.
Determination of phosphorus
is done using wet decomposition of organic samples followed by spectrophotometric detection or via
oxygen-flask combustion method or using the Anton Paar Microwave Induced Oxigen Combustion
(MIC) for the decomposition of organic samples
and subsequent by Ion chromatography analysis using a Dionex IC system. Oxygen-Flask Combustion
MIC 835 Titrando Ion Chromatography 5.
Water Determination by Karl Fischer is
done using 835 Titrando System consisting of a volumetric Karl Fischer titrator with built-in buret drive for volumetric
Karl Fischer titrations. Karl Fischer Accuracy CHN
weight percentages are accurate and reproducible to within ± 0.3%, for a very
pure sample. Halogens, S and P weight percentage are accurate and
reproducible to within ± 0.5%. Microbalance Accurate
weighing of samples is a prerequisite for organic elemental analysis since
results are presented on a weight percent basis. A microbalance is an
instrument capable of making precise measurements of weight of objects of
relatively small mass: of the order of a million parts of a gram. Mass spectrometry 6.
High Resolution Mass
determination, separation and Qualitative and Quantitative MS analysis by
LC(UV)MS/MS Analysis using Agilent 6520 QTOF analyzer. High-resolution LCMS/MS
Quadruple Time Of Flight is an compound
identification tool which allows distinguishing peaks separated by only small
m/z values and provides excellent mass accuracy < 2ppm for MS and
< 4 ppm for MS/MS also allows selective
detection of desired compounds in the presence of interferences. Liquid
Chromatography Mass Spectrometry enables chromatographic separation,
identification and quantification of
compounds in a wide range of masses. Ionization of the samples occurs in Dual
Electro Spray Ionization source which is suitable for compounds that can have
a charge induced and for multiply charged compounds such as: proteins,
peptides, oligonucleotides. The samples for this
analyzer have to be soluble in polar well vaporizable solvents. Q-TOF
LC/MS Sample submission:
Please
provide as much information as possible about your sample, and what element
you wish to determine. Give the formula and draw the structure or at least an
indication. Deduce your %CHN, O, S, F, Cl, Br, I,
and P content. It is essential that you provide your name, phone number,
e-mail address, your advisor's or company name, and purchase order or account
number for billing purposes. You
should provide a bare minimum of 5 milligrams for CHN analysis. You will need
to provide an additional amount if you require further analysis in addition
to CHN. In cases where you have a very small amount of sample, it is possible
to do one CHN run with only 1 mg. Analysis duration: CHN
and MS results are normally completed within 3 working days, but this can
vary depending upon sample load and instrument problems. Halogens,
S and P analysis are usually finished within 5 working days. You
can ask for urgent analysis, at an additional cost. Results Results
can be picked up at the laboratory or sent to you via e-mail or regular mail. Contact: For
further information please contact the lab team: Lab
E-mail: micro.chem@mail.huji.ac.il Lab manager: Dr. Carina Hazan E-mail: carinah@savion.huji.ac.il Dr. Ella Jakob E-mail: ella.jakob@mail.huji.ac.il Rachel Benzicry E-mail: rachelbe@savion.huji.ac.il Tel:
(972) 2-6585606 Fax:
(972) 2-6584934 |
Address:
The
analytical Chemistry
Lab, The Hebrew University of
Jerusalem
Edmond J. Safra Campus
Los Angeles building, room 114
Givat Ram, Jerusalem, 91904,Israel